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Centrifuge the supernatant again for 30 min at 18,000 rpm at 4 °C to get a clear soluble lysate

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In adult life, compared to early life, there is a downregulation of mitochondrial genes (increasing the likelihood of mitochondrial mutations) and an upregulation of genes associated with the innate immune response, the response to proteotoxicity, the response to oxidative stress, and purine biosynthesis